Exact(15)
This could cause differential PCR amplification of the mixed template present in the faecal samples [47].
Direct sequencing of PCR products for all but the ITS region failed in V. longisporum, indicating the presence of a mixed template population.
To avoid formation of chimera PCR products due to the presence of mixed template, the following guidelines proposed by Beser et al. [73] were implemented.
The amplicons produced from this mixed template pool were cloned and sequenced.
These mixed template DNAs were treated with bisulfite and then used to generate SEPT9- and TMEFF2-specific PCR products.
To test the ability to detect two different NDV variants in a single sample, a series of mixed template PCRs was prepared.
Similar(45)
First, samples containing mixed templates at 104 copies per assay were prepared (for mix compositions see supporting Table S4) and run on microarrays.
The mixed templates are composed of CTAB and glucose with a molar ratio of 1/1.
Various templates have their advantages and disadvantages, and recently there is a growing interest in the application of mixed templates.
Open image in new window Fig. 3 XRD of MgO-rich MgAl2O4 prepared by using mixed templates.
In fact, both the single templates and mixed templates can be employed during the co-precipitation to synthesize MgO-rich MgAl2O4.
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