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This genetically engineered strain can be used for l-ribulose production by fermentation using mixed sugars of glucose and l-arabinose.
No obvious inhibition of xylose utilization by glucose was observed in the mixed sugars of glucose and xylose for all genotypes examined.
The supplementation of glucose restored the cellular growth, and the growth rates of Z. mobilis 8b in mixed sugars of glucose and xylose (RMGX and RMGXAc) were same as those in RMG and RMGAc.
A new microarray was designed including both coding sequences and intergenic regions to investigate the acetate stress responses of Zymomonas mobilis 8b when using single carbon sources of glucose or xylose, or mixed sugars of both glucose and xylose.
An understanding of the toxic effects of acetate on the fermentation microorganism and the efficient utilization of mixed sugars of glucose and xylose in the presence of hydrolysate inhibitors is crucial for economic biofuel production.
A recently engineered, TAG-producing strain of R. opacus MITXM-61 exhibited a xylose-fermenting feature with the capability of utilizing mixed sugars of glucose and xylose at high concentrations in genuine lignocellulosic hydrolysate and producing a large amount of intracellular TAGs as a precursor for lipid-based biofuels [ 56].
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In the exponential phase, the effect of mixed sugars on gene expression in the presence of acetate is more like that of glucose than that of xylose with more differentially expressed genetic features (154 features, Table 1, Additional file 3: Table S2-11).
However, xylose utilization was inhibited in both media containing xylose or a mixed sugar of glucose and xylose, although the performance of 8b was better in mixed sugar than xylose-only media.
The consistent upregulation of ZMO1682 in Z. mobilis 8b in response to acetate was independent of sugar source of glucose, xylose, or the mixed sugar of RMGX, indicating its critical role in resistance to the weak acid of acetate.
Interactions among acetate upregulated genes and downregulated genes for Z. mobilis 8b grown in mixed sugar of RMG4X4 with at least twofold significant increase (data from Table S2-7) using the STRING precomputed protein-interaction database.
Lignocellulosic biomass is recognized as a sustainable source of mixed sugars for fermentation to second generation biofuels and biomaterials [ 1].
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