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The biochemical conversion process requires higher pretreatment and enzyme costs, has low fermentability of the mixed sugar stream (C 5), and generates inhibitory soluble compounds (Munasinghe and Khanal 2010).
(2) A lignin press with counter-current washing was added after hydrolysis to separate lignin and unreacted insoluble solids from the dilute mixed sugar stream [ 1]. (3) A triple-effect evaporator system was added to the model, with heat input specified to achieve 50%% water in the sugar syrup.
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Given the ability of A. succinogenes to utilise hemicellulose-derived sugars such as xylose and arabinose, multiple bench-scale SA production studies have been conducted with this microbe using mixed sugar streams enriched in xylose, including from corn stover hydrolysate [ 30], straw hydrolysate [ 31], and sugar cane bagasse [ 32].
The final glucose concentration from the two-stage hydrolysis would be ~7.5%, obtained by mixing the concentrated sugar stream (~10%) from the first hydrolysis prior to the washing step with the sugar stream from the second stage hydrolysis (~5%).
Another way to integrate streams from 1G and 2G earlier in the process is to mix the sugar streams in the evaporation stage.
Stream integration was simulated by combining the distillation streams from the 1G and 2G processes in the same unit (Scenario I, Figure 3) and by mixing the sugar streams from 1G and 2G in the evaporation step (Scenario J, Figure 4).
The highest total FFA concentration from the mixed sugar culture reached 2.96 g/L by ML190 pXZ18Z).
KF7M-16 utilized glucose first in the mixed sugar medium.
K. ohmeri strains, studied here showed promising mixed sugar fermentation potential with enhanced xylose utilization.
The fermentation kinetics were evaluated for simultaneous mixed sugar fermentation.
The use of lignocellulosic sugar syrup is much easier than the pretreated biomass since the lignocellulosic sugar stream can be easily blended into the existing process.
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