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Moreover, the specific consumption rate of total sugar during the mixed sugar fermentation was the same as when either glucose or xylose was fermented separately as a sole carbon source.
K. ohmeri strains, studied here showed promising mixed sugar fermentation potential with enhanced xylose utilization.
Ethanol was the major product of glucose and xylose fermentation though trace amounts of xylitol and acetic acid were also detected during mixed sugar fermentation.
In a mixed sugar fermentation it was found that the An14g04280 overexpression strain, in contrast to the parent control strain, has a preference for D-galacturonic acid over D-xylose as substrate.
We have previously shown that the co-culture of multiple strains is preferred over the culture of a single strain for mixed sugar fermentation (Unrean and Srienc 2010; Suriyachai et al. 2013).
In addition, ethanol yields from xylose during the mixed sugar fermentation (0.30 g g−1) were higher than ethanol yields from xylose as a sole carbon source (0.21 g g−1).
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In mixed sugar fermentations with recombinant Saccharomyces cerevisiae strains able to ferment D-xylose and L-arabinose the pentose sugars are normally only utilized after depletion of D-glucose.
In contrast to single sugar fermentations, such as corn-based or sugarcane-based ethanol fermentations, mixed sugar fermentations present significant challenges for cost-effective production of the target products.
The effect of the increased uptake of D-galacturonic acid on the preferential uptake and metabolism of substrates has been assessed by mixed sugar fermentations and enzyme activity measurements.
The recombinant Escherichia coli strains with homologous or cyanobacterial ppc overexpression and ldhA, pflB, ptsG mutations were constructed, and the mixed sugar fermentations were carried out with the prominent strain SD121.
This agrees well with previous studies on both pure glucose and mixed sugar fermentations with other strains of S. cerevisiae, where ethanol yields were reduced at increased pH in the presence of acetic acid as a consequence of improved anaerobic cell growth (and associated glycerol production) (Casey et al. [2010]; Taherzadeh et al. [1997]).
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