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Samples taken from direct cultures, D-xylose in the mixed substrate medium serves as an inducer of the xlnD promoter.
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The sequential substrate uptake observed in mixed substrate growth medium, however, suggest that it is a result of strict CCR.
Thus, the initially simple environment becomes a mixed-substrate growth medium where metabolic interactions can take place.
Subsequently, vesicles were rapidly separated from the substrate medium by filtration on mixed ester filters (0.45-μm pore size; Millipore Corp., Bedford, MA) and washed with 3× 2 ml of PBS.
After 30 s, uptake of MeAIB was terminated by adding 2 ml of ice-cold PBS and rapidly separating vesicles from the substrate medium by filtration on mixed ester filters (0.45 μm pore size, Millipore Corporation) and washed three times with 2 ml of PBS.
In mixed substrate fermentation, significant xylose utilization is initiated by Scheffersomyces (Pichia) stipites once glucose concentration in the medium is below 20g/ll (Agbogbo et al. 2006).
Novel cultivation strategies such as mixed substrate feeding are discussed.
The substrate consumption rates in the five single carbon-limited cultures correlate with their apparent substrate uptake order in mixed substrate culture.
The initial pH was read immediately following particle suspension of thoroughly mixed substrate.
The effect of mixed substrate fermentation on n-butanol production appears to be strain dependent.
The C : N ratio of the mixed substrate was found to be 1.26.
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