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MrBayes was run with a mixed substitution model and a Γ4 distribution.
A mixed substitution model was set by allowing model jumping between nine fixed-rate amino acid models.
BI trees were reconstructed using the program MrBayes v. 3.0b4 [ 80] with a mixed substitution model and a Γ distribution of substitution rates with 4 categories.
Therefore, BI trees for this marker were computed with MrBayes v.3.0b4 [ 55] with a mixed substitution model and a Gamma distribution of substitution rates with 4 categories.
For Bayesian analysis, a mixed substitution model with gamma law (four rate categories) and a proportion of invariant sites were used.
Analysis for both Acsl3/4 and Acsl1/2/5/6 amino acid sequences were performed under a mixed substitution model, with two parallel runs with 1 million generations, each with four chains one cooled and 3 heated.
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Inferred site covariation patterns were used then to guide the application of mixed substitution models in subsequent phylogenetic analyses.
Peptide sequences of IFITM1, 2 and 3 proteins were analysed, set with priors (prset aamodelpr = mixed) and substitution model (lset rates = invgamma Ngammacat = 8).
Misof et al (2007) [ 10] published a large phylogeny of Hexapoda using 18S rDNA and applying mixed DNA/RNA substitution models.
In the final step, we try to improve the analyses by fitting biologically realistic mixed DNA/RNA substitution models to the rRNA data.
Thus, we modeled non-stationary processes combined with the application of mixed DNA/RNA substitution models in a Bayesian approach using the PHASE-2.0 software package [ 63] to provide a better fit to our data than standard substitution models [ 60, 64].
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