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Individuals were assigned to source populations using mixed stock analysis techniques employing Bayesian mixture modeling [40] using the software program cBayes.
Mixed stock analysis was performed using SPAM v. 3.7 [ 21].
Released fish are not genetically tagged, and the large number of genetic combinations arising from a free-mating culture system makes targeted genetic identification of hatchery signatures (e.g., familyprinting or mixed stock analysis; Pella and Milner 1987; Letcher and King 1999) in the F1 or later generations computationally intensive, if not impossible.
High-throughput microarrays containing assays for thousands of SNPs are becoming available for a number of non-model organisms [ 1- 3], and being used more frequently in ecological and evolutionary studies, including population genetics studies e.g. [ 4- 7], QTL identification e.g. [ 8], parentage determination e.g. [ 9- 11], and mixed stock analysis e.g. [ 12- 15].
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The experimental design and sampling strategy critically affect the reliability of a mixed-stock analysis.
Mixed-stock analysis of conglomerating green turtles from TCI's, have shown the mitochondrial haplotype CM-A3 as the most common, a widespread haplotype in Caribbean rookeries, including Costa Rica (Tortuguero), Florida and Mexico [ 30].
To estimate the proportion of contribution of Arab, Oriental and British Native horses to Thoroughbred horses, we performed mixed-stock analysis of allele frequencies, using SPAM v. 3.7 [ 21].
For quantitative analysis, six different concentrations of a mixed stock solution containing ten analytes were injected.
FUNDAMENTAL stock analysis takes you only so far.
The series concentrations of mixed working solution were prepared by diluting the mixed stock solution with methanol.
Generally, mixed stock analysis methods such as implemented in the programs oncor (S. Kalinowski, available at: http://www.montana.edu/kalinowski) and CB ayes (Neaves et al. 2005) have been applied to mixed stock fisheries samples for both assessing the stock composition of the sample and assigning individuals to the population of origin (e.g. Beacham et al. 2005).
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