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Whole games that involve mixed stages of this sort are games of imperfect information, however temporally staged they might be.
Mixed stages of worms, grown on NGM plates, were extensively washed in M9 buffer.
We have sequenced, mapped and assembled 581,365 long and 507,719,814 short reads from RNA of intact and mixed stages of the first 7 days of planarian regeneration.
Based on the predicted gene sequences, the agrin-specific primers (Table 1; Fig. 1) were used to amplify overlapping fragments of each of the predicted ORFs and of a putative common transcript from cDNA reverse transcribed from RNA isolated from mixed stages of worms.
Nematode cDNA was prepared by RT (reverse transcription) using isolated total RNA from mixed stages of N2 worms and a RT kit with an oligo dT) primer (Promega).
Finally, the posteriormost region of vasa RNAi testioles contained irregular groups of cells at mixed stages of late spermatid and spermatozoon differentiation (Fig. 6L), rather than the perfectly synchronised cysts of late spermiogenic stages seen in controls (Fig. 6G).
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Both Raman and infrared experiments have demonstrated that when the tension is removed, a mixed stage of conformations is observed.
The selection was performed depending on the time of eruption; early eruption enables the values to be used as independent prediction variables from the early mixed stage of dentition.
In a standard immunoprecipitation the starting material was 1g of mixed stages floral tissue of four week-old plants, grown under long day conditions.
We tested the supernatant of mixed stage cultures of all ten species in a Congo red-polysaccharide interaction assay and found complete correlation with the transcriptome data [ 3, 23].
sbs03: Subtractive library of a mixed stage population of clone 1B4 (tester) vs. mictic females with resting eggs of clone 1B4 (driver).
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