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sbs04: Subtractive library of mictic females with resting eggs as tester vs. mixed stage population of clone 1B4 (driver).
sbs01: Subtractive library of a mixed stage population from a combination of cloned and non-cloned cultures (tester) vs. mixed stage population of a clone 1B4 (driver) sbs02: Subtractive library of a mixes stage population of clone 1B4 (tester) vs. a mixed stage population of a combination of cloned and non-cloned cultures (driver).
sbs03: Subtractive library of a mixed stage population of clone 1B4 (tester) vs. mictic females with resting eggs of clone 1B4 (driver).
A mixed stage population of BC3129, rol-3 (s1040 ), was harvested at the permissive temperature of 15°, and the worm suspension was incubated in 2 μg/ml TMP in the dark for 1 hr.
Instruments such as the COPAS Biosort (Union Biometrica) may allow for enrichment of transgenic animals through automated separation of fluorescent and nonfluorescent animals; transgenic iL3 S. stercoralis larvae were selected from a mixed stage population using this instrument (Junio et al. 2008).
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All experiments were repeated under identical conditions using mixed stage populations of P. murrayi.
Mixed stage populations of N2, xpa-1, nth-1 and nth1;xpa-1 were reared at 20°C on HT115 DE3 -seeded HT115 DE3 -seeded plates per replicate, 3 replicates per strain) until the nematones had cleared the plates of food.
Two studies that performed large scale sequencing of small RNA libraries cloned from mixed stage populations of C. elegans have provided initial material for grouping and classification of candidate endo-siRNAs [ 5, 6].
Fusion constructs were made from restriction enzyme digested purified PCR products that were amplified from C. elegans cDNA made from total RNA that was reverse transcribed using an oligo-dT or a random primer from a mixed-stage population of wild type N2 worms.
Then, we made lysates from the mixed-stage population of worms, reacted them with phosphine-Flag, and analyzed the resident glycoproteins for the presence of azides using standard Western blot techniques (1, panel c).
For determining the temperature-sensitive period of mir-35 -41(nDfecundityundity, a mixed-stage population of mir-35 -41(nDf50 ) animals containing a lag-2:: GFP reporter (qIs56 ) marking the distal tip cells was shifted to 25° for 12 hr.
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