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Exact(4)
We mixed spores with or without TEV sites in ScpA and followed their outgrowth with time-lapse microscopy.
Raper and Thom [ 22] first mixed spores of D. discoideum and D. purpureum and reported the absence of intermediate phenotypes, which was in accordance with our results, but does not preclude chimerism.
We verified that this manipulation actually impacted relatedness among spores within fruiting bodies as follows: To assess the average degree of relatedness within fruiting bodies for each treatment, we mixed spores of all eight clones at equal frequency and plated the mixed populations at the experimental high and low relatedness conditions (500 and 50,000 spores/plate, respectively).
We mixed spores of the eight clones in equal proportions and started two sets of twelve replicate populations by plating 500 spores/plate for high relatedness and 50,000 spores/plate for low relatedness on SM plates in association with a bacterial food source.
Similar(56)
To investigate specificity, the biosensors were exposed to mixed spore solutions containing B. anthracis Sterne, Bacillus cereus and Bacillus megaterium spore strains.
In addition, a mixed spore suspension was made up to examine inter-strain competition.
The group received bags of waste mixed with spores and learned how to manage a mushroom house.
After crushed and ground, the dried sporophore of puffball was mixed with spore powder.
They then mixed D. discoideum spores with spores of D. mucoroides, a species that is as equally distant phylogentically as D. purpureum [ 14].
Briefly, three-day-old larvae in groups of ten were fed with 500 μl of the spore suspension in mixed diet (~10 spores per larva), and kept at 33° C under 97% RH in 6-well culture dishes.
The fungal spores, mixed in oil, have been trademarked Green Muscle.
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