Another silk fabric of 2.45 g weight was mordanted using iron sulfate (referred to as iron-mordanted silk in the following) and then dyed with the mixed dye solution following the same procedure as above.
Six standard dyes were dissolved together in 300 mL DMSO with concentrations 0.016 g/L for indigotin and indirubin, 0.033 g/L for berberine, palmatine, alizarin, and purpurin to make a standard mixed dye solution, following Ahn et al. (2013).
Briefly, serum samples were mixed with sulfanilamide solution following incubation at room temperature.
The isolated E. coli bacteria are then mixed with 6 μM Syto-9 or 30μMM propidium iodide solution following the procedure shown in the LIVE/DEAD Baclight bacterial viability kit.
The mixed solution became clear following digestion, and it was then cooled down at room temperature.
The Ca bound organic carbon (Ca-OC) and iron/aluminum bound organic carbon (Fe/Al-OC) were extracted using 0.5 M Na2SO4 and a mixed solution of 0.1 M NaOH and Na4P2O7 following the outline of Xu and Yuan [22].
Briefly, cultures were rinsed with PBS following treatment and 50 μL of mixed solution of 2 μM Calcein AM and 2 μM ethidium homodimer-1 was added directly to cells.
It can be explained in the following: Under the synergistic effect of liquid paraffin, the mixed solution rapidly permeate across the stratum corneum within 5 min, it leads to the dehydration of top layers of tissue.
In the following step, the wafers were etched in a mixed solution containing H2O2, C2H5OH, H2O, HF, and HAuCl4 with a typical ratio of 10 4 4 2 1, resulting in pores.
A volume of 300 nl of the mixed solution was stereotaxic microinjected into the LA region.
