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The typical preparation route was as follows: 1.70 g tetrabutyl titanate was added into a mixed solution consisting of 45-ml dehydrated ethanol and 5-ml oleic acid, under stirring.
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X-ray photoelectron spectroscopy (XPS) analysis showed that the corrosion films formed in the mixed solution consisted of zinc oxide (ZnO), zinc hydroxide (Zn(OH 2) and zinc hydrozincite (Zn5 CO3 2(OH)6).
Then, SiNW and SiNH arrays could be achieved by immersing the Ag-covered Si substrate into a mixed etchant solution consisting of HF and H2O2, with the catalysis of either the Ag mesh or the Ag nanoparticles, respectively (Figure 1c,f).
Droplets of mixed lipid stock solution consisting of POPC (Avanti Polar Lipids) and PtdIns(4 P (Matreya, Pleasant Gap, PA, USA) were spotted on coverslips, dried and rehydrated.
The protein concentration was measured in the supernatants of each sample, and 1 µg of protein was mixed with the substrate solution consisting of 5 mM hippuryl-L-histidyl-L-leucine, 0.9 M NaCl and 0.4 M borate buffer at pH 8.3 to a final volume of 75 μl.
SWCNTs with anionic surfactant sodium dodecyl sulfate (SDS) are first mixed with an aqueous wormlike micellar solution consisting of cationic surfactant cetyltrimethylammonium bromide (CTAB) and organic salt sodium salicylate (NaSal).
0.1 µl of the protein solution (19.6 mg/ml) was mixed with 0.2 µl of well solution consisting of 0.2 M ammonium nitrate and 20% (w/v) PEG 3350.
Next, the samples were mixed with 20 μL of a reaction solution consisting of 50 mmol L−1 Hepes NaOH (pH 7.4), 4 mmol L−1 PEP, 200 mmol L−1 KCl, 10 mmol L−1 MgCl2, and 1.2 units of pyruvate kinase.
The pH was adjusted to 7.5 by the addition of several drops of a mixed solution, which consisted of 1.2 M NaOH and 0.8 M Na2CO3.
A solution of peptides was filtrated via Millipore (Millipore ZTC18M096) and mixed with the same volume of a matrix solution consisting of saturated a-cyano-4-hydroxycinnamic acid (CHCA) in 50 % ACN containing 0.1 % TFA.
The addition of the mixing term in this simple additive equation yields better predictions for mixed solutions mainly consisting of the structure-making ions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com