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We inferred these data to be consistent with a common size variation in the length of the ovine SPRN ORF, with animals with mixed sequence electropherograms representing heterozygotes for size variation, and the boundary between coherent and mixed sequence in the electropherogram representing the boundary of the indel heterozygosity.
The product was too weak to be detected in mixed sequence in either cerebellum or cortex.
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Direct sequencing yielded an unambiguous sequence without evidence of mixed sequences in 7 specimens.
Analysis of 3 18 plasmid clones for each fragment showing mixed sequences in repeat regions identified 2 or 3 variants as follows: region B in strains M2282 and M2321, region EF in 64.0 and 64.1, region G in strains M2321 and 199.1, and region JKLM in strains M2282, M2300, and clinical specimens 199.0 and 199.1.
In DNA from two of the three T2 plants, all eight clones from the same plant contained the same DNA sequence lacking the BAR gene, distinctive from the mixed sequences in leaves of induced primary transformants (T1 plants).
Direct sequencing of 16S amplicons also revealed mixed sequence traces in a number of the Osedax individuals that we examined.
Gel mobility shift assays with the mixed sequence 25mer oligomer used in these studies indicate that pRPA has a relative increase in ssDNA binding affinity over that of RPA.
We found that homopolymeric guanine oligomers are not digested by BAL 31 and that the presence of short dG tracts in mixed sequence oligomers hinder BAL 31 enzyme activity.
Each allele of a complex locus with mixed sequence tags was scored in each parent and DH individual using RFAPtools.
The amount of DNA added is critically important: if too much DNA is added, the beads will bind multiple DNA molecules resulting in mixed sequence on each bead and as a result will be unreadable; if not enough DNA is added, the emPCR will not deliver enough Sequence Capture Beads for efficient sequencing.
To obtain initial affinity values for the DNA complexes of different compounds, thermal melting experiments were performed with hairpin oligomer duplexes containing a pure AT sequence (5′-AATT-3′) and a mixed sequence (5′-ATGA-3′ in the preferred 5′-ATGAT-3′ context).
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