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The ORF A sequence of this strain could not be analyzed because of mixed sequence data.
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Inferring clonal substructure from mixed sequencing data and flow-sorted subpopulations using SciClone.
Given the mixed sequencing data, i.e. the short reads produced in this work and the publically available red clover EST sequences (mostly Sanger sequences), this constitutes a hybrid assembly.
The promise for bioinformatics is that public genome data, mixed with proprietary sequence data, clinical data from previous drug efforts and other stores of information, could unearth clues about possible candidates for future drugs.Unlike banking, bioinformatics offers big challenges for data mining because of the greater complexity of the information and processes.
In mixed male and female sequence data, Y sequences should have one-quarter the coverage of autosomal sequences [ 29].
The development of specific software to assist the identification of mixed sequencing electropherograms using the sequence data deposited in the public databases must be encouraged in this respect.
Better sensitivity and specificity can be achieved through a combination of (1) using mixed alignable and non-alignable sequence data and (2) combining evidence from multiple sites within an intergenic region.
Secondary analysis included comparisons of each of the vaccine formulations (RTS,S/AS01A and RTS,S/AS02B) versus control as well as an analysis taking into account mixed alleles by including all available sequence data (data not shown; analysis did not alter results as there were few such infections in our dataset).
Direct sequencing of amplified ovine genomic DNA revealed a number of samples with unusual electropherograms (not shown), wherein coherent sequence data diverged into a "mixed" output comprising two co-existing sequences.
Sequence data obtained from a mixed population of tumor and stromal cells often masks signals, and we found this to be true for our tumors as well.
For both sites, we did not detect any mixed sequence populations when analyzing the gene-specific amplicons (data not shown).
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