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Concentration changes after E+P use were identified by testing the hypothesis that the mean of the log-ratios across the (up to 5) mixed samples is zero, by using a weighted moderated t statistic [ 24] implemented in the R package LIMMA [ 25]: the log-ratios were weighted by the number of quantified peptides for each protein, and a matrix of weights was included in the linear model.
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The flowability of the mixed samples was tested immediately after mixing following ASTM D6103 (2004).
This series of mixed samples was analyzed using the MRM method established above.
After 4 h, the mixed samples were stained with PI and analyzed by FACS.
In the natural runoff experiments, volume proportional mixed samples were collected after each runoff period during 1992 1999.
After 4 h, the mixed samples were stained with PI, and the killing of target cells were analyzed by FACS.
The mixed samples were then extracted with 1.5 mL of dichloromethane: n-Hexane (1 2), by vortex mixing for 2 min.
In order to dissolve the PMMA powders, the mixed samples were placed in a dark room for several days.
The mixed samples were added to the wells of 96-well cell culture plates (each concentration sample was performed in triplicate) and incubated at 37 °C.
The mixed samples were left for 48 h to cure and subsequently remixed to achieve a homogenous mix prior to compaction at Modified AASHTO level of compaction.
On the average, the moisture content of the maize cobparticles, C. pentandra and the mixed samples were 9.00%, 13.27% and9.21%, respectively.
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