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Chromatin was prepared from the mixed sample and immunoprecipitated exactly as previously described [14].
To estimate the proportion of normal cells (
Both mixed sample and microarray were kept at 55°C until the hybridization step.
There were 31 948 transcripts that overlapped between the mixed sample and midgut transcriptomes.
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Thus, the mixed samples and the river sand certainly increased the packing beyond the limit.
The array could be used to detect individual Fusarium species in mixed samples and in environmental samples.
Limitations of prior studies include weak designs, mixed samples, and a lack of valid and reliable evaluation tools.
The activation energy dependence for diffusion of sodium ions on the partial dry density was different between the mixed samples and the pure montmorillonite samples.
Then, PCR products were generated from two single-sources, mixed samples and artificially degraded DNA samples using a multiplex PCR system, and were prepared for sequencing on the MiSeq system through construction of a subsequent barcoded library.
Third, we expand the number of component cell types quantified in mixed samples, and we validate the method on an independent test set of those cell types.
Urine was collected for 24 h, uniformly mixed, sampled, and then stored at −80 °C until subsequent analyses.
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