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In mixed relatedness groups, non-sibling nymphs were discernable from full sibling nymphs via a morphological marker, white eyes.
The effect sizes (Cohen's d, {95%CI}) of relatedness on sperm viability were 0.04 {−0.10, 0.19} for full sibling groups and 0.12 {−0.34} 0.34} for mixed relatedness groups.
In the second experiment, we reared a second generation of experimental crickets produced as above, but raised in mixed relatedness groups.
Mixed relatedness groups consisted of fifteen newly hatched full sibling nymphs, and fifteen newly hatched non-sibling nymphs of mixed parentage, making a total of 30 individuals.
We do this by raising crickets in either full sibling groups (high competition between close relatives), or mixed relatedness groups (low competition between close relatives).
This result was obtained in our first experiment when experimental males were raised in full sibling groups, and in our second experiment when experimental males were raised in mixed relatedness groups (Table 1, Figure 1).
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We expect that individuals raised in mixed-relatedness groups should be more likely to display preferential behaviour towards their relatives, than individuals raised in full sibling groups.
Sequences were clustered into relatedness-groups (OTUs) by average-linkage clustering, using the application sortx [25].
Phylogenetics is the study of the evolutionary relatedness among groups of organisms.
These taxonomists used the underlying morphology, or physical structures of organisms (such as flowers, shells, and bones), to illuminate the relatedness of groups of living things.
I speak to mixed groups.
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