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However, assembling of a set of mixed reads from different species to form contigs is a bottleneck of metagenomic research.
'Mixed' reads in 454 sequencing are defined as four consecutive positive nucleotide flows for a given read.
Mutations marked with *indicate mixed reads in Sanger (& NGS), where the wt variant can also still be detected.
For the experiments in which both RNAs were mixed, reads were mapped against an artificial genome formed by the union of the chromosomes of both species.
The remainder either failed to be amplified from the meadow fescue genotype, or, when sequenced, produced mixed reads indicative of multiple product amplification.
For some samples their exploitation may be tedious as the assemblies from mixed reads of low divergence may produce chimeric scaffolds, in particular for conserved, short contigs (Mavromatis et al. 2007).
Similar(52)
The use of mixed read data sets is more difficult than simulations, probably due to library duplications, genome structural rearrangements, sequencing errors, and mapping errors, etc.
To mine allelic variants from mixed sequence reads (mixed alleles) at the same position, we first designated the alleles as A1, A2, A3 etc.
The index closed at a nominal high on Friday despite a mixed reading on the labor market, which showed that hiring slowed in July, but the unemployment rate ticked lower to 7.4 percent.
Sport England figures released this week made for mixed reading for those who believed the Games would result in a sportier nation but in women's boxing the effect has been marked – the number of 16-to-19-year-olds 16-to-19-year-olds 16-to-19-year-olds 16-to-19-year-olds 16-to-19-year-olds
Roger is very good at understanding the appeal of a mixed read; other people at the time probably thought I was a bit trivial or mainstream in my interests, but he was happy enough to bring that into the paper alongside the more heavyweight political opinion and harrowing news.
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