Comb area and T levels were measured prior to and after treatments, so we tested for treatment effects on changes over time using Generalized Linear Mixed Models (Mixed procedure in SAS).
To adjust for repeated measurements in all other analysis we included animal ID as a random factor in a mixed model using the MIXED procedure in SAS.
These EQ-5D values were analysed based on a linear mixed model using the MIXED procedure in SAS 9.1 (SAS Institute, Cary, NC, USA).
In an additional analysis, we used the linear mixed-effects models (MIXED) procedure in SPSS, which allows an unequal number of repetitions and a better handling of missing values.
Finally, for data measured at three timepoints (GPAQ scales, TPS and Pathways to Care scale), mixed model analysis was employed, using the MIXED procedure in SPSS (consistent with methodology carried out in the primary outcomes article [ 14]).
A linear mixed effects model was performed on the relative concentration by using MIXED procedure in SAS 9.2 (SAS Institute Inc ,Cary, NC) to evaluate effect of Tanner stage and gender on these metabolites.
Two-level regression model with random intercepts was used to investigate the contribution of district's characteristics to spatial variation in amenable mortality (MIXED procedure in SPSS).
Data collected over the last 2 to 3 d were analyzed using the MIXED procedure in SAS SAS Institute Inc.., Cary, NC).
The model was fitted using the MIXED procedure in SAS (Littell et al., 2006).
We fitted the model using the Restricted Maximum Likelihood (REML) with unstructured variance covariance, using the mixed procedure in SAS [55].
Analysis was implemented using the MIXED procedure in SAS for Windows, Version 9.1.
