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In general, the accuracy varies with coronary arterial plaque composition: volumes of non-calcified and often also mixed plaques are frequently underestimated, whereas the volume of calcified plaque is often overestimated in CT [20].
Adiponectin may modulate early stages of atherogenesis thus mainly affecting the development of non-calcified or mixed plaques.
Approximately twice as many mixed plaques were identified by DADA-PCR as by flanking primer PCR (Fig. 2B, Table 1).
Following co-electroporation, mixed plaques were identified, and a homogenously pure mutant was readily recovered (Table 3).
Our data indicate that adiponectin may reduce the number of non-calcified and mixed plaques without affecting stable calcified plaques.
Plaques were recovered and examined by flanking and DADA-PCR (Fig. 3A) revealing mixed plaques at frequencies of 5 12% and 20 36%, respectively (Table 2).
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Right Clearly visible mixed plaque in the coronary artery.
c CT coronary angiography demonstrates an obstructive coronary stenosis due a mixed plaque in the proximal LAD (C1) and a chronic total occlusion in the mid LAD (C2).
In contrast, a mixed plaque that is readily identified by flanking primer PCR is likely to require the screening of far fewer secondary plaques.
The proportion of mutant plaques was expected to be directly related to the ratio of mutant to wild-type genomes in the primary mixed plaque.
Although we predicted lysA to be essential, the mutant presumably grows in the mixed plaque through assistance of wild-type helper phage.
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