Sentence examples for mixed mutants from inspiring English sources

An insertion vector containing a selectable ura4+ marker and a random barcode was used to generate a collection of 10,000 fission yeast insertion mutants stored individually in 384-well plates and as six pools of mixed mutants.

As an effort to further support genome-wide studies in S. pombe, we generated a barcode-tagged S. pombe insertion mutant library which is available as pools of mixed mutants for parallel analysis and in the form of 384-well mutant arrays for genetic screens on individual mutants.

When wild-type and mutant type were mixed, mutant DNA could be repeatedly detected in the percentage of 1∼10% (Figure 4E).

We mixed mutant plasmid with corresponding wild type plasmid at various ratios and amplified the mixed plasmid as a template of PCR using paired primer sets for mutational analyses.

Assay sensitivity was therefore estimated for each mutation, by LOD using mixed mutant IDH DNA with WT genomic DNA at 5 mutation percentages i.e. 2, 5, 10, 15 and 20%.

There were relatively few mixed mutant infections, with the highest prevalence of mixed infections found at dhfr 59R, where there were 4.3% mixed infections among episodes in the continued co-trimoxazole arm and 6.3% mixed infections among episodes in the discontinued co-trimoxazole arm.

To compare mutant and wild-type chromosomes directly, we mixed mutant and wild-type cells before spheroplasting, and the two types of nuclei were distinguishable by the presence in wild-type cells of TetR-GFP foci associated with centromere-linked Tet operators.

27 Specimens were classified as wild type or pure mutant, or mixed (both mutant and wild type alleles detected in the same specimen).

Among the tested mutants, intriguingly, a mixed mutation of H3-K14,18Q/K14,18R allowed normal growth but increased the age-associated autophagic activity above that of wild-type cells (Fig. 1).

DNA from this heterozygous mutant and a homozygous non-mutant sample were mixed to give mutant:non-mutant DNA ratios of 1 0, 1 1, 1 2, 1 3, 1 4 and 1 5.

To determine the sensitivity of the COLD-PCR melting curve assay to detect low quantities of mutant allele in WT background we adopted two complementary strategies: (1) we mixed KRAS-mutant cell line DNA with KRAS-WT peripheral blood leukocyte DNA, and (2) KRAS-mutant FFPE tumor DNA with KRAS-WT FFPE tumor DNA at mutant:WT ratios of 50/50, 20/80, 10/90, 5/95, 2/98, and 1/99.