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We found that the amphiphilic drug indomethacin enhances immiscibility of saturated and unsaturated lipids and induces the formation of gel-phase domains in mixed model membrane bilayers.
The formation of tightly packed domains was further confirmed by characterizing Indo-induced changes in the fluidity and detergent resistant properties of the mixed model membrane.
Using a series of fluorescent probes in a fluorescence resonance energy transfer (FRET) study, we found that Indo induced separation between gel domains and fluid domains in the mixed model membrane, possibly by enhancing the formation of gel-phase domains.
Here we explored the effect of Indo on the nature of the segregated domains in a mixed model membrane composed of dipalmitoyl phosphatidyl-choline (di16∶0 PC, or DPPC) and dioleoyl phosphatidyl-choline (di18∶1 PC or DOPC) and cholesterol that mimics biomembranes.
Further, CTxB and antigen binding have been shown to alter the membrane organization by inducing lipid phase separation in previously mixed model membrane systems and by affecting the local environment of key membrane proteins in living cells.
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In this study, we examined the effect of a potent NSAID, indomethacin (Indo), on the phase behavior of a mixed model bilayer mimicking biological membranes composed of saturated and unsaturated phosphatidylcholine (PC) with or without cholesterol (Chol).
Generalized linear mixed model.
Multi-locus mixed model.
linear mixed model analysis.
Generalized additive mixed model.
Variables were analysed using a mixed model.
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