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#Mixed model group vs. variable interaction.
Panel b shows the PaCO2 changes over time (mixed model: group vs. PaCO2 interaction, P = 0.913; timepoint vs. PaCO2 interaction, P = 0.311; timepoint vs. group interaction, P = 0.899).
Panel c shows the SBE evolution over time (mixed model: group vs. SBE interaction, P = 0.02; timepoint vs. SBE interaction, P < 0.001, timepoint vs. group interaction, P < 0.001).
Panel d shows the HCO3 changes over time (mixed model: group vs. HCO3 interaction, P = 0.401; timepoint vs. HCO3, P = 0.607; timepoint vs. group interaction, P = 0.664).
Panel a shows the pH changes over time (mixed model: group vs. pH interaction, P = 0.089; timepoint vs. pH interaction, P < 0.001; timepoint vs. group interaction, P = 0.003).
Panel e shows the phosphate changes over time (mixed model: group vs. phosphate interaction, P = 0.565; timepoint vs. phosphate interaction, P = 0.450; timepoint vs. group interaction, P = 0.531).
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The change in perceived pain (0 10 scale) will be evaluated using a repeated measures linear mixed model with group, time and group by time interaction as independent variables.
The mixed model includes group, age and group Χ age of the family member as fixed effects and a random family effect with an unstructured correlation matrix.
The change in frequency and duration of events with excessive physical workload will be evaluated using a repeated-measures linear mixed model with group, time and group by time as independent variables.
For secondary outcomes we will use a mixed model with group as a fixed factor.
A mixed model with group as a fixed factor will be used for the other outcome measures.
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