In males, mortality rates were similar between stud and dud treatments when measured in a mixed sex milieu (their laboratory environment), but when measured in a single sex milieu, males from the stud treatment outperformed males from the dud treatment, having reduced rates of baseline mortality Fig 4A and senescence (Fig. 4B; Table 3).
The recognition of AERD as a combined Th2-/Th1-like disease prompted us to query the role of this mixed cytokine milieu in driving the AERD phenotype.
Similarly for longevity and the mortality parameters determining it, there was no evidence of any differences between stud and dud treatments when measured in a mixed sex milieu (the environment to which the populations are adapted).
Consequently, the collective inclusion of the genes in cluster 5 in the SigB-mediated stress response may fine-tune the stress response for competition with other microorganisms in a mixed microbial milieu.
In line with the results for mean life span, Gompertz mortality parameters for females did not differ significantly between stud and dud treatments, but did differ between single and mixed sex milieu (Fig. 3; Tables 2 and 3).
If the salaries were not exactly Wall Street caliber, well, they came with a milieu that mixed cultural swagger with pure Manhattan high life.
Sources of variation include treatment (stud vs. dud ancestry), milieu (single vs. mixed sexes), and their interaction.
Differences in mortality parameters (α and β) were tested using a multivariate general linear model (MANOVA) with treatment (stud vs. dud), milieu (single vs. mixed sex), and their interaction as fixed effects.
Differences in mean life span were tested using a general linear model (ANOVA) with treatment (stud vs. dud), milieu (single vs. mixed sex), and their interaction as fixed effects.
The present findings clearly suggests that, on vaccination with Bm-iPGM, a correct milieu with a mixed type of Th1/Th2 immune response accompanied with innate immunity was maintained which was efficient in providing significant degree of protection against establishment of B. malayi in the immunized host.
