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The oil sludge-derived mixed microorganisms were suspended in MS medium and subsequently inoculated into the fresh MS medium supplemented with 2% yeast extract (YE) (see "Materials and methods").
Meanwhile, different P4 inclusion levels [0 (control), 2, 20, 40, 80, and 100 ng/mL in culture fluids] were incubated in vitro for 6, 12, 24, 36, 48, and 72 h together with rumen mixed microorganisms grown on a maize-rich feed mixture (maize meal Chinese ryegrass hay = :1) with an aim to determine microbial P4 transformation into testosterone and ADD.
Hypersensitivity pneumonitis was first described in dairy farmers exposed to aerosol from stored, moldy hay containing mixed microorganisms.
Kang et al. [ 10] treated mixed microorganisms with mutagens NO2−, diethyl sulfate (DES), UV, and their combinations, and the best one increased the content of Cu2+ by 101.4% in 20 days of leaching compared with the control culture on chalcocite-leaching.
For the treatment of landfill gas containing H2S and NH3, they can be easily treated by two immobilized cell biofilters (ICB) with different microorganisms in series or single ICB column with mixed microorganisms, as shown in our previous studies [ 2, 3].
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A gas system fitted with an oxygen sensor and with a regulated atmosphere of nitrogen (80%) and carbon dioxide (20%) was used in the preparation and incubation of the anaerobic and mixed microorganism consortium.
Thus, a mixed microorganism culturing system consisting of three types of microorganisms which form a minimum food chain system as a closed ecosystem (chlorella as the producer, bacteria as the decomposer, and rotifer as the consumer) was taken for the subject, on which to research the universal characteristics of ecosystems.
T. longibrachiatum enzymes acted synergistically with enzymes from mixed rumen microorganisms in degrading soluble cellulose, xylan, and corn silage.
This work was designed to assess the nutritional influence of pre-incubation in saliva on in vitro fermentative activity when some tanniniferous shrubs were incubated in batch cultures of mixed ruminal microorganisms.
Two in vitro experiments were conducted to study the effects of supplemental non-fiber carbohydrate (NFC) and fermentation pH on rate and extent of forage neutral detergent fiber (NDF) digestion by mixed ruminal microorganisms.
Batch cultures of mixed rumen microorganisms were used to study effects of different concentrations of fumarate on fermentation with various N sources (ammonia as ammonium bicarbonate, casein amino acids, casein peptides, gelatin peptides) and feeds (bermudagrass hay, mixed diet of 60% bermudagrass hay plus 40% concentrate) for 6 and 24 h, respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com