Sentence examples for mixed medium was from inspiring English sources

We then counted and diluted the cells to 1500 cells/1 ml DMEM/F12+10% FBS, and 1 μl of the mixed medium was added to each well of 96-well plates containing 170 μl serum-free DMEM/F12 medium.

The mixed medium was then poured into Petri dishes.

The xylitol yield under the xylose/glucose mixed medium was higher than that under the xylose medium.

Considering that glucose and xylose coexist in corncob hydrolysate, C. tropicalis W103 using a xylose/glucose mixed medium was investigated under anaerobic and aerobic conditions.

But when the mixed medium was used for two weeks, no network formation or expansion was obtained in the substitutes in this study (data not shown).

And the Pu-18-NMGA PS 1 (0.8 25  μg/mL) or corresponding amount of the PS-GNPs conjugates 2a– 2e (constant amount of the PS 1) in 100  μL of the mixed medium was added in each well.

A549 Cells (1 × 10 cells/well) in 100  μL of the mixed medium were placed in a 96-well plate and incubated for 48 h (37°C, 5% CO2).

This suggested that the transcriptional profile of cells during glucose fermentation phase in the mixed sugar medium was nearly identical to those growing in glucose alone medium.

Since the metabolic activity during the xylose fermentation phase in mixed sugar medium was similar to that in xylose alone medium, it is interesting to observe that when the transcription profile of GX2 was compared to that of X, 682 genes were differently expressed, with 375 upregulated and 307 downregulated genes (Table S5, in the supplementary material).

In our study, the expression level of the genes related to tryptophan degradation (BNA1, BNA4, BNA7, ARO8) decreased when the transcriptional profile of xylose fermentation stage in the mixed sugar medium was compared to that of the glucose fermentation stage, corroborating the involvement of tryptophan in response to ethanol stress.

The mixed conditioned medium was then supplemented with 10 m M β-glycerophosphate and 50  μg ml 1 ascorbic acid (both from Sigma). 5 × 10 SaOS-2 cells were seeded into each well of a 24 well-plate.