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Microsatellite data from eight polymorphic loci (Supplementary Data Table S1) pointed to intermediate selfing rates in wild populations (i.e. mixed mating) (Table 2 lines 30 and 31).
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The effective number of pollen donors, which is the reciprocal of correlated paternity, varied from 1.001 in HLG to 2.049 in GML using the Ritland mixed mating model and from 1.661 in HLG to 2.314 in GML using TWOGENER (Table 5).
The average correlated paternity varied from 0.488 ± 0.086 in GML to 0.999 ± 0.164 in HLG with the Ritland mixed mating model, and from 0.432 ± 0.051 in GML to 0.602 ± 0.026 in HLG using TWOGENER (Table 5).
The fixation index (F) was highly variable among loci in each population (Table 1); averaged across loci for each species, it ranged from 0.230 to 0.631, which is consistent with a mixed mating system in Rhodiola.
If correct, the Colombian populations we sampled may have a mixed mating system, characterized by a low but significant degree of outcrossing, evidenced by the relatively low inbreeding-coefficient compared to literature (Additional file 10: Table S4).
MLTR software was used assuming a mixed mating system.
B. orellana displays a mixed mating system affected by the agronomic system.
Possibly more open vegetation (savanna) may stimulate mixed mating systems while selfing may be an ecological response to more closed vegetation types (rainforest).
C. heterophylla has a mixed mating system, i.e. a combination of outcrossing and selfing [49].
Those authors posited that reproductive assurance in unstable environments is achieved through mixed "mating" systems [40].
The mating system of weedy rice populations was calculated from 5527 progeny using a multilocus mixed mating model [36], based on six highly polymorphic SSR loci.
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