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4.0 mL of ultrapure water was added and mixed manually, for 1 min.
Finally, the NaOH pellets were incorporated, and the geopolymer mortar was mixed manually for an additional 10 min.
First, the fly ash and limestone sand were dry mixed manually for 1 min, and then the H2O and Na2SiO3 solution were added and mixed for 2 min.
Following by 10 min of incubation, 0.2 mL of chloroform was added, and samples were mixed manually for 20 s and then incubated for 4 min.
These were mixed manually for 2 min and then transferred to a multi-vane paddle mixer (Model: MX-25, Unitech, New Delhi, India) for further mixing for 2 min.
Sixty-three ml salt-enriched MHB was added to the sponges; mixed manually for 30 seconds and 100 μl was spread-plated onto a chromogenic MRSA selective medium (Chrom-ID™ MRSA; BioMerieux, Marcy l'Etoile, FR) after which the plates were incubated overnight 18-200 h, 37°C).
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The composting reactors were mixed manually by wearing sterile gloves for obtaining a homogen mass before taking the samples.
Measured quantities by weight of dry shale rock dusts with varying percentages (0, 3, 6 and 9%) of cement were mixed manually in a sample tray for various geotechnical tests.
The shredded MSW and dried faecal sludge were then mixed manually in a 2 1 mix ratio.
For scaffolds fabricated in the presence of cells, microspheres were mixed manually with DMEM (Gibco UK) containing 2 × 10 NIH-3T3 fibroblasts using a spatula at a ratio of 1 1 (microspheres to DMEM).
With computerized boards still in the offing, the sequence's dozens of tracks had to be mixed manually.
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