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Illustration of the mixed (left) and the mixed-mixed (right) case of LOOCV.
For transient transfection, after 24 h, 1 μg of purified plasmid DNA resuspended in 50 μl of D-MEM without FCS and 2 μl of Lipofectamine 2000 (Invitrogen) in 50 μl of D-MEM were mixed, left at RT for 20 min and added to each well of cultured cells.
Thirty ml of 5% oxalic acid was added to the mortar, the contents mixed, left for 10 mins.
The solution was mixed, left for 15 min at room temperature, and centrifuged at 6,000 × g for 15 min. After decanting the supernatant, 1 ml of cold 70% ethanol was added to the pellet, mixed and centrifuged at 6,000 × g for 10 min. The supernatant was decanted, and the tube was placed upside down on a paper towel and dried for 5 min at 37°C.
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He mixed lefts and rights with hooks and jabs, occasionally switching to a southpaw stance while landing quick, hard hits on head and body.
The reaction mixture was mixed and left to stand for 10 min.
Both the solutions were then mixed and left in the shaker overnight at room temperature.
The polar lipid monoolein (MO) and poly ethylene glycol), PEG, of different molar mass (1500, 4000 and 8000) were melted, mixed and left to solidify at room temperature.
The tubes were mixed and left stirring for 15 min at room temperature to enable the thiol exchange to occur.
The components were mixed and left on air at approximately 20°C till the solvent is evaporated (approximately 12 h).
To be specific, aqueous solutions of HAuCl4 and CTAB were first mixed and left undisturbed at room temperature for several minutes.
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