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However, a combination of lamivudine and siRNA M184V was very effective in inhibiting replication of both wild-type and variant M184V viruses in mixed infection experiments.
Competitive indices (CIs) derived from mixed infection experiments were analyzed with Student's t-test.
The mixed infection experiments employed a ∼1∶1 mixture of mutant and wild type strain.
Surprisingly, mixed infection experiments showed that the ΔB mutant was severely attenuated also in Fg-deficient mice (Figure 3C).
Mixed infection experiments in Fg-deficient mice were performed as described above, except that spleens and livers were harvested after 20 h.
(Note that this dose of wild type bacteria was twice as high as that used in the mixed infection experiments described below, explaining the lethality).
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Interestingly, a mixed set of results were obtained from infection experiments in chicken and mice.
Infection experiments were performed using mixed egg clutches from wild type AB×TL or transgenic UAS KAEDE/MPEG1 GAL4 strain zebrafish [ 15].
Recent laboratory experiments with rabbits with a mixed infection of Schistosoma japonicum parasites of different ages confirmed the effectiveness of a combination therapy.
According to the M&M, most of the infection experiments were done in hybrid maize, while the mutants used are in various (and often mixed) genetic backgrounds.
For the simultaneous infection experiments, iPSC-Mac (4 × 106/animal) and PAO1 (0.2 × 105 CFU/per animal) were resuspended in PBS and mixed in a total volume of 60 µl.
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