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Localization of MEIG1 was further examined in mixed germ cells isolated from adult wild-type mice.
To further verify its localization, mixed germ cells were isolated from adult mice, and these cells were double stained with anti-PACRG antibody and anti-α-tubulin antibody.
Mixed germ cells from Meig1-deficient mice were also double stained with an anti-MEIG1 antibody and an anti-α-tubulin antibody.
Deparaffinized tissue slides and isolated mixed germ cells were permeabilized with 1% Triton X-100 for 5 min at 37°C, blocked for 1 h at room temperature with 10% goat serum in PBS.
The protocol consists of the dissociation of the seminiferous tubules and epithelial cells of the testis followed by separation of mixed germ cells by unit gravity sedimentation on the basis of their size.
With quantitative RT-PCR (qRTPCR) analysis for selected autosomal and X chromosomal genes, we found a specific derepression of X-chromosomal gene activity for RNA isolated from mixed germ cells and purified spermatids from Hr6b knockout mice [ 39].
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However, primary spinal mixed germ cell tumors are extremely rare.
Non-seminomatous germ cell tumours include yolk sac tumours, endodermal sinus tumours, embryonal carcinomas, choriocarcinomas and mixed germ cell tumours, which present as large masses typically with marked heterogeneous attenuation.
Mixed germ cell tumours contain more than one germ cell component.
The imaging findings of malignant transformation may be similar to those seen in mixed germ cell tumours.
The imaging features of mixed germ cell tumours are variable due to the diversity of their components.
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