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The bead pellet was mixed with 1 mg of protein of the cellular extract and mixed gently overnight.
One hundred microlitres of the DNA- LipofectamineTM LTX complexes was added directly to each well and mixed gently by rocking the plate back and forth.
The soil was mixed gently to distribute the sludge homogeneously.
Then the solutions were mixed gently and stirred until the two solutions mixed properly.
The phases were mixed gently for known time and then left to separate.
The erythrocyte suspension of mice (30 μL) was added to each tube and mixed gently by upending.
Antibody solution (50 μl) was added and mixed gently by shaking the plate manually and incubate for 10 min.
Supernatant was shifted in another tube and 300 μl chloroform was added and mixed gently by inversion.
The samples were again vortex mixed gently for 1.5 min and then cold centrifuged for 10 min at 10000 rpm.
After incubation, the plate was mixed gently, and 0.05 mL of this suspension was smeared on the glass slide.
Antibody solution (50 μl) was added and mixed gently by shaking the plate manually and incubated for 10 min. at room temperature.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com