The morphology of the structures formed by the MCF7 cells cultured in mixed gels containing collagen and rBM proteins was different from that observed in collagen gels regardless of whether or not RMF were present in the culture.
Much greater increase in the intensity of the excimer emission are observed for the mixed gels containing 7 compared to those with 6, consistent with the longer-lived excited state of this isomer.[ 38] The increase in excimer emission is more pronounced in mixed gels of 5 compared to 3 or 4 indicating greater dissolution of the gels of 5 by acetate.
However, the addition of MTGase was able to improve the textural properties of surimi gel containing gelatin or mixed gelatins to some extent.
Then the PCR products were denatured for 5 min at 100°C, mixed with formamide-containing stop buffer, and subjected to electrophoresis on 4% polyacrylamide gel containing 8- M urea in an ABI Prism 377-18 DNA sequencer (Applied Biosystem).
Samples were mixed with loading buffer and electrophoresed on 8% SDS-polyacrylamide gel containing 0.1% gelatin.
Conditioned media was collected after 24 h stimulation, mixed with non-reducing sample buffer, and electrophoresed on a polyacrylamide gel containing 0.1% (w/v) gelatin.
The amplicons were denatured for 5 min at 100°C, and mixed with formamide-containing stop buffer, and then subjected to electrophoresis on 4% polyacrylamide gel containing 8 MM urea in an ABI Prism 377-18 DNA sequencer (Applied Biosystem, Foster City, CA, USA).
After completion of the primary and the nested PCR, a 5 μL aliquot of the PCR products was pipetted out from each tube and mixed with 2 μL loading fluid for electrophoresis in a 2% agarose gel containing ethidium bromide.
Serum-free conditioned medium samples were mixed with sodium dodecyl sulfate (SDS) sample buffer and loaded onto a 10% polyacrylamide gel containing 0.1% gelatin.
The selected fractions (see Bioassay guided selection below) were mixed with CH2Cl2 until they were not sticky and were then mixed with silica gel, evaporated to dryness and loaded on top of the silica gel containing column.
