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The yield of the mixed extract was 11.25% % (w/w).
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AFE, VME, and the mixed extract were dissolved in normal saline and administrated orally to the mice (200 mg/kg/day).
The mixed RNA extract was subjected to Solexa sequencing analysis at the Beijing Genomics Institute (BGI; Shenzhen, China).
The mixed protein extract was then purified by using a 2-D Clean-Up Kit (GE Healthcare, Uppsala, Sweden), and the purified protein sample was dissolved in rehydration solution [7 M urea, 2 M thiourea, 4% (w/v) CHAPS, 0.002% (w/v) bromophenol blue] supplemented with 2% (v/v) 3 10 NL IPG buffer (GE Healthcare) and 5.4 mg/ml dithiothreitol.
An amount of 0.5 g of plant extract was mixed with 5 mL 0.9% saline at physiological pH, mixed by inversion, vortexed and allowed to incubate on a rocker for 20 minutes.
Briefly, 50 µL of egg lipid extract was mixed with 450 µL hexane.
The water-GT extract was mixed with 20 g/L of sodium alginate powder (Promova Biopolymer Norway), and sterilized.
The extract was mixed with petroleum ether at a ratio of 5 2 (v/v) at room temperature.
To measure the radical scavenging activity, 40 μl of the root extract was mixed with 1.96 ml of ABTS-.
Then, as described in par.2.4, the extract was mixed with unaggregated AuNPs and analysed in RAS mode.
An 80 µL of methanolic extract was mixed with 1.92 mL DPPH solution, and absorbance was immediately noted at 515 nm.
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