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An EF-G2 fusion protein (GFP-CG31159) that lacks the TP shows a similarly mixed expression pattern (Figure 6E).
We observed a mixed expression pattern amongst the low-grade to high-grade samples.
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Lines showing mixed expression patterns (see Figure 1B-F) made up 68% of CK controls (47 lines) and 29% of NRSE drivers (18 lines).
CK-derived lines tended to have mixed expression patterns, with multiple cell types and tissues labeled for more images of CK lines).
In the current work, we analyzed RNA extracted from PBLs of ALS patients and control subjects, thereby reducing some of the complexity of mixed expression patterns generated by RNA from reticulocytes, granulocytes, monocytes, thrombocytes and plasma, normally present in whole blood.
In view of the mixed-stage expression pattern and because the level of Oct4 mRNA/protein was lower in rat XEN-P than ES cell lines (Fig. 2E, F), we suspected that our rat XEN-P cell lines were heterogeneous.
The third group of transcripts shows unique mixed differential expression patterns under the tested conditions (Fig. 6, columns with blue and red highlighting).
ACs showed membranous expression of E-cadherin and displayed a mixed but exclusive expression pattern of CK8 and CK14, but lacked expression of vimentin and SMA (Fig. 5A; supplementary material Table S1).
Like the adenocarcinomas from Wcre Trp53 F/F females, lobular carcinomas showed a mixed CK8 and CK14 expression pattern, but did not express vimentin nor SMA (supplementary material Table S1), indicating that mILC cells display epithelial properties.
In contrast, removal of the C-tail reverts to a mixed cytosolic and nuclear expression pattern (Figure 6D).
The fact that early but not late C5 cells exhibited a mixed morphology and gene expression pattern prompted us to investigate whether the segregation into the endoderm and trophectoderm cell types occurs upon the plating of rat blastocyst on feeder cells in mouse ES medium.
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