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Depending on the genotype of each SNP in the two individuals whose DNA was mixed, dilution series of samples with different allelic ranges were obtained for the ten SNPs, as specified in Table 1.
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When visualizing these mixed dilutions immediately after printing, we observed equal fluorescein spot intensity among all spots in the dilution series, which is consistent with the manufacturers specifications that the TeleChem SMP3 printing pin deposits approximately 6 nl of probe volume per contact with the glass surface (image not shown).
The carbon particles are generated onsite by the Carbon Particle Generator (CPG) and are mixed with dilution air prior to entering the SPHER.
Because sperm in the BC and SP are mixed [27], dilution occurs between newly received sperm and aged sperm already stored in the female, resulting in lower viability of the sperm within the female than in sperm transferred by males.
For quantification, mixed plasmid dilution series ranging from 6 × 10 to 6 × 10 gene copies/reaction were included in each run.
PCR efficiency (E = 10(−1/slope) – 1) was estimated by testing at least ten replicates of ten-fold mixed plasmid dilution series in different runs, and was acceptable between the values of 0.90 and 1.10.
The quantitative range was determined from the range of ten-fold mixed plasmid dilutions that gave optimal linearity, efficiency, standard deviation and coefficient of variation between replicates.
Alternatively, the desired strain was mixed with dilutions of bacteriophage lysate at 37°C, incubated for 30 min, and incorporated in a soft agar layer on solid medium.
Unfavorably, this increase in CMC suggested that the stability of mixed micelles toward dilution might be decreased compared with plain micelles formed by mPEG-PLA.
Cells (100 μl) were then mixed with 1 1 dilution of (100 μl) 0.4% trypan blue solution.
V o2 was measured continuously using the mixed expirate argon dilution method that allows the mass spectrometer alone to measure metabolic gas exchange and ventilation volume.
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