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In this study, At. ferrooxidans strains (FOX1 and YTW) were found to be the dominant strains followed by L. ferriphilum strains (BN and YTW315) in all mixed cultures systems.
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Specificity was assayed in pure and mixed culture systems using DNA extracted from 28 different fungal strains as PCR template.
The present study highlights the opportunity and power of applying proteomics to mixed culture systems for which metagenomic sequences are available.
Other benefits of mixed culture systems include tunability and increased resistance to environmental stress [ 12, 13].
The pH of the medium is known to regulate the shift to solventogenesis during the fermentation of sugars [ 7]; the effect of low pH in the inhibition of methanogenic archaea is also recognized and could be potentially used as a selective pressure in mixed culture systems.
The mixed culture system involved S. cerevisiae and C. shehatae with the aim of utilizing hexose and pentose sugars, respectively.
Electron microscopy was, however, challenging in this mixed culture system and clearly identifiable neurite/fibroblast interfaces were rare.
The mixed enzyme and mixed culture system using 1% (w/v) pretreated wild grass yielded 2-fold higher ethanol than single enzyme single culture system.
This assumption may be valid for the mixed culture system, provided that the substrates are utilized by a common enzyme system.
This exhibits that usage of mixed culture system involving economically feasible mixed recombinant enzymes, GH5 cellulase and GH43 hemicellulase in the present SSF studies offers a comprehensive choice of bioethanol production.
A 2-fold increase in ethanol titre (23 g·L−1) (Table 3) was obtained in lab scale bioreactor on scaling up the shake flask SSF (11.2 g·L−1) (Table 3) with mixed enzyme mixed culture system using 5% (w/v) wild grass.
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