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Lastly, to check whether enhanced BA utilization in PP9 is only induced in presence of PA01 and E. coli JM 101 or whether this phenomenon is more generalized and stands true for other bacterial species as well, we monitored BA degradation profile of PP9 in mixed cultures containing a Gram positive bacillus (Lactobacillus sp).
The TPS10 product TAB can be detected in the open headspace around lox2/3 plants in mixed cultures containing a lox2/3xTPS10 plant.
Table 2 presents results of analyses of deltamethrin biodegradation by pure strains of epiphytic and neustonic bacteria and by mixed cultures, containing 25 strains.
Populations were either monocultures, or mixed cultures containing a single TPS10-expressing plant at the center and plants of the same defense- and HIPV level at the edges (WT around TPS10, and lox2/3 around lox2/3x TPS10, Figure 2B).
In mixed cultures containing enrichments of polyphosphate and glycogen-accumulating organisms (PAO and GAO), different P/O ratios have been reported depending on the carbon source used for culture enrichment, namely acetate and propionate.
We observed multiple significant indirect effects of TPS10 expression in populations, as mentioned above; and two of these were strongest in mixed cultures containing only one TPS10-expressing plant.
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The mixed cultures contained mainly Arcanobacterium pyogenes, Bacteroides spp., Fusobacterium necrophorum, Peptostretococcus indolicus and Escherichia coli.
The tanks in the foreground are used for pretreatment and for delivering a mixed culture containing many different organisms that break down biomass.
PN occurs via AOB according to Eq. 1, but activated sludge is a mixed culture containing both AOB and NOB.
Results of these tests indicated that the type strain isolate was a mixed culture containing an uncharacterized A. cryaerophilus strain.
To confirm that fluorescence was specific to infection of M. smegmatis, fluoromycobacteriophages were used to infect a mixed culture containing approximately equivalent amounts of M. smegmatis and an Escherichia coli strain expressing the fluorescent reporter gene DsRed (Figure 3).
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