In addition, we will regard participants as a random effect and will use Gaussian mixed-model estimations (SPSS MIXED command).
All models are formalised in Additional file 1: Appendix B. The mixed command is the successor of the xtmixed command.
The checkpoint was under mixed command - Ukrainian police who had defected from Kiev to Crimea's pro-Russian autonomous government, heavily-armed soldiers wielding AK-47 rifles and a group of Cossacks - one of whom was ready to talk.
A linear mixed model (mixed command in Stata) was used to find the annual age decline in lung function parameters (mL/year) for current and former hairdressers.
The PROC MIXED command of the SAS program was used to determine the effect of tea compared with the control.
Nucleotide substitution models were unlinked across partitions, and a reversible-jump MCMC over the space of all GTR sub-models was run for each of them ("nst = mixed" command in MrBayes [ 36]).
Instead of selecting a substitution model a priori, we used the "mixed" command to sample across the GTR model space in the MCMC analysis [ 56], with the addition of I + Γ to all partitions.
The homogeneity of the SD on the output was tested Post hoc using a likelihood ratio test estimated by a proc mixed command in SAS (version 9.0), in order to analyse if inter-instrument reliability changed over time.
The models used were similar to the kind that might be fit in a multi-center study, i.e. mixed models incorporating a random care group effect (PROC MIXED command in the SAS® 9.2 Software), which was considered most suitable given the possibility of 'residual heterogeneity' [ 30].
The molecular evolutionary history of the Shiva superfamily toxins was reconstructed using Bayesian inference as implemented in MrBayes version 3.2.1 [ 89], using lset rates = invgamma with the prset aamodelpr = mixed command, which enables the program to optimize between the nine different amino acid substitution matrices implemented in MrBayes.
This was done by adding the statement "RANDOM REPCROSS (SIRE*DAM);" to the PROC MIXED commands, where "SIRE" and "DAM" refer to the line of the sire and dam, and "REPCROSS" is a class variable.
