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Consistent with this hematopoietic phenotype, A/B ECs generated mostly myeloid CFUs consisting of both granulocyte/monocyte (GM) and macrophage (Mac) types, as well as larger erythroid burst-forming units (BFU-E) and mixed colonies containing both erythroid and myeloid elements (Mix) (Fig. 5I).
Colonies were scored by morphology after 12-14 dassmallsmall, primitive erythroid (CFU-EryP), macrophage (CFU-Mac), granulocyte/monocyte/macrophage (CFU-GM), large, burst-forming erythroid (BFU-E), or mixed colonies containing both erythroid and myeloid elements (CFU-Mix).
However, as illustrated in Figure 1c, in the assays of the dissociated regenerated structures, we also noted the presence of many "mixed" colonies (containing both CK14+ and CK18+ cells within the same colony).
Of these colonies, 72% were astrocyte-generating, 18% were oligodendrocyte-generating and 10% generated mixed colonies containing both astrocytes and oligodendrocytes.
Colony collection occurred late in the season (September – November), when usurpation would have been complete, so we did not find mixed colonies containing workers of both species.
Cells expressing that phenotype had high cloning efficiency in culture from a single cell, generating mixed colonies containing luminal cells and myoepithelial cells.
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In adherent culture on a collagen substratum, ER+ cells generated progeny bearing only luminal markers (CK18, MUC1, EpCAM) while ER– cells or cells sorted on viability alone generated mixed luminal-myoepithelial colonies containing both CK14+ and CK18+ cells as well as pure luminal (EpCAM+) and myoepithelial (CD10+) colonies.
The class of clones defined as "T+ mixed with Sox2+" refers to colonies containing T Bra)+ single positive cells mixed with Sox2+ single positive cells (example shown in the 5th row in Fig. 3C) while the group defined as "T+/Sox+ mixed with T-Sox2 " describes clones containing T Bra)+ OR Sox2+ single positive cells mixed with double negatives (example shown in bottom row in Fig. 3C).
Colonies containing 50 cells were counted.
Subterranean termites form colonies containing thousands of individuals, and maintain these colonies by consuming wood and other materials containing cellulose.
Twelve stably transformed colonies containing each construct were selected.
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