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G. mellonella larvae were reared on baby mixed cereal (1200 ml) (H.J. Heinz Company, Canada) supplemented with glycerol (119 ml), water (98 ml), sucrose (100 ml) and multi-vitamins (Enfamil, Poly-vi-sol) (1 ml) at 28 30°C with 50 60% relative humidity and a 12L:12D light cycle as described previously [71].
This study developed an assay that can be used to detect and quantify the waxy content of mixed cereal samples.
At seven months, mixed cereal was consumed by a significantly greater proportion of subjects and gradually replaced rice cereal in prevalence.
Use of this qPCR assay to analyze mixed cereal products demonstrated that it could accurately detect the waxy content of samples containing both waxy and non-waxy sorghum.
In contrast, much higher values for the immunoassay were found in samples TR-D100/D-W-153, TR-D100/D-W-163 and the mixed cereal samples (4 7-fold).
This study demonstrates that the presence and quantity of waxy-grain sorghum or waxy-grain sorghum powder in mixed cereal products can be evaluated using quantitative real-time PCR with waxy allele-specific primers.
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Breakfast is a bowl of mixed cereals - Weetabix, All-Bran and Alpen all together.
Mixed cereals showed the maximum fungal load with 3 × 103 colony forming units/g.
The co-contamination analysis showed that 44, 40 and 50% of the mixed cereals, maize and barley samples were concurrently contaminated with AFs and OTA.
Our findings demonstrate that cereals used as animal feeds are generally contaminated with variable levels of mycotoxigenic fungi and mycotoxins, with maximum levels being present in mixed cereals and maize samples.
Aflatoxins (AFs) and ochratoxin A (OTA) were detected in 94 and 44% of mixed cereals, 70 and 40% of maize, 40 and 60% of wheat and wheat bran samples, respectively.
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