Sentence examples for mixed cell suspensions from inspiring English sources
Fresh bone marrow cells and in vitro cultured polySia-NCAM-positive SH-SY5Y neuroblastic cells were mixed together in different ratios, and the mixed cell suspensions were labeled with the polySia-binding fluorescent fusion protein.
After the indicated times of co-incubation, thoroughly mixed cell suspensions were analyzed for ECTV by plaque assay.
This method allows for highly efficient transfection of DNA or siRNA into isolated primary cell populations or within mixed cell suspensions as well as into other cell populations that are considered difficult to transfect.
Mixed cell suspensions of lymph node and spleen were plated in 24-well plates in complete IMDM supplemented with hIL2 (10 U/ml) and stimulated with irradiated (1600 rad) B16OVA cells (5*104 cells/well) that had been treated overnight with r-IFN-γ (50 U/ml) to up-regulate MHC class I presentation.
The cell separation technique allows at least a 10-fold increase in sensitivity to detect tumour cell specific mRNA in mixed cell suspensions.
PBMC were obtained by Ficoll-Hypaque density centrifugation as described above, and CD14+cells isolated from the mixed cell suspension.
The mixed cell suspension was plated into a culture flask and incubated for 15 minutes to allow most of the MEFs to attach, leaving mainly rat iPSCs suspended.
In fact, our results show that macrophages, DCs and B cells have higher β-lactamase activity than T cells in both purified cells and mixed cell suspension, suggesting that these three cell types are more sensitive to anthrax LT.
In a standard print run, the tips were primed, rinsed in running water and vacuum dried twice after each loading and printing step, The cell suspension was kept on ice to prevent the gelation of collagen before printing and agitated gently just prior to placement on the printing robot to ensure a uniformly mixed cell suspension.
For both screens, 125 µL of mixed cell suspension was run per sample.
injections in the right flank of 7 × 10 DU145 cells/mouse in 200 μl of a 1 : 1 mixed cell suspension and Matrigel.
