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Experiments using samples containing mixed cell populations with known molecular phenotypes validated this mathematical un-mixing method for the RNA sequencing (RNAseq) transcriptome, targeted (selected reaction monitoring) proteome, and global (shotgun) proteome data with a <16% average error.
The present results warrant further studies on the use of engineered helper cells, including those expressing other growth factors injected as mixed cell populations.
The spontaneous transition of Epstein-Barr virus (EBV) from latency to productive infection is infrequent, making its analysis in the resulting mixed cell populations difficult.
By using a CP, we demonstrated the sensitive detection of EGFR gene mutations in purified genomic DNA from mixed cell populations.
We demonstrate that randomly-seeded mixed cell populations undergo rapid condensation and self-organization into discrete epithelial and endothelial structures that are mechanically robust and stable during long term culture.
The harvested cells from most tissues contain a mixture of cell types and usually the downstream processing starts and ends with mixed cell populations, achieving only enrichment and not purification.
A two-term exponential model for mixed cell populations, a model based on a Weibull distribution of resistances within the bacterial population, a sigmoidal equation also justified by the existence of a resistance distribution, and a purely empirical equation were used to fit the observed survival curves.
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Our analysis is based on the replicator equation describing the frequency dependent evolutionary dynamics of three well-mixed cell populations (Hofbauer and Sigmund, 1998).
We therefore established tumor allografts by injecting mixed-cell populations of EC-vGPCR and EC-vCYC/vFLIP cells in athymic nu/nu mice (Fig. 6).
To understand the regulatory networks underpinning cellular dynamics both purified and mixed-cell populations need to be studied.
The DEPArray™ is a new technology that allows the selection and isolation of single cells from mixed-cell populations and our results show we were able to select uncontaminated CTCs by combining the CellSearch and DEPArray™ systems.
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