Sentence examples for mixed cell lysates from inspiring English sources

We mixed cell lysates with 15 mL scintillation fluid.

This topological requirement may explain why β3-homophilic binding did not occur in mixed cell lysates (Fig. 4 C).

It is important to recognize that binding detected with mixed cell lysates could occur in either cis or trans.

A total of 5,808 array spots were arranged on each slide, including the spots corresponding to positive and negative controls prepared from mixed cell lysates or dilution buffer, respectively.

These interactions were confirmed by co-immunoprecipitation of Myc-tagged ESCRT-III proteins from mixed 293T cell lysates that contained One-strep-flag (OSF -tagged ULK3.

For pull-down of immune complexes, 25 μl of GFP-TrapM® Beads (Chromotek, Germany) were washed twice with ice-cold dilution buffer, mixed with cell lysate and incubated for 2 h at 4°C under agitation.

Briefly, 100 µg of the GST control protein, GST-CXCR2-311-355 (full length CTD), GST-CXCR2-311-330 (first half of the CTD) and GST-CXCR2-331-355 (seCTDd half of the CTD) were washed in the binding buffer and mixed with mammalian cell lysates overexpressing the HA-LASP-1, HA-LIM, HA-LIM-NR and HA-NR-SH3 proteins for 1 h at 4°C.

To assay the linearity in the context of a cellular extract, increasing amounts of pure PtdIns3 P were then mixed with HeLa cell lysates.

To address this question, because Sav and dRASSF repress each other's expression and dRASSF has reduced affinity for phosphorylated Hpo, we mixed separate Kc cell lysates expressing a kinase-dead form of Hpo (HpoKD-Flag), Sav-HA, and HA-dRASSF and performed IPs after the proteins were allowed to bind overnight.

Cell lysates mixed with loading buffer (Tris Glycine SDS Sample Buffer (Invitrogen) with 5%% 2-mercaptoethanol) were loaded onto 15%% polyacrylamide gels and electrophoresed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

The supernatant of the cell lysates was mixed with buffer containing acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) as a substrate peptide and incubated at 37 °C for 3 h.