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Furthermore, the use of purified epithelial populations, rather than mixed cell isolates, may alter estimates of stem/progenitor cell potential.
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In similar early experiments, mixed cells isolated from the adult Hydra were shown to segregate and form separate tissues (Gierer et al. 1972).
Indeed, high-throughput miRNA expression profiling has been previously performed using mixed cell populations isolated from skeletal myoblasts [ 26, 27].
Mixed cell populations were isolated from mammary gland tumour extracts of either neu or neu/S100A4 transgenic mice (Davies et al, 1996), as described previously (Davies et al, 1993).
To determine whether viable hPSCs could be isolated from mixed cell populations using lectins, a mixture of WA09 hESCs and fibroblasts was sorted using UEA-I-linked magnetic beads.
Localization of MEIG1 was further examined in mixed germ cells isolated from adult wild-type mice.
PBMC were obtained by Ficoll-Hypaque density centrifugation as described above, and CD14+cells isolated from the mixed cell suspension.
Therefore, it is necessary to have an efficient method to isolate iPS cells from mixed cell populations.
To our knowledge, this is the first report showing that lectins can be used to isolate and remove hPSCs from mixed cell populations.
In order to further characterise this heterogeneous cellular population of HBM, we looked for lineage specific markers in this mixed cell population at the mRNA and protein level in freshly isolated, uncultured WCP from HBM.
The relative difficulties of isolating large numbers of primary islets, and their mixed cell populations, have made insulin-producing cell lines an important tool in beta cell research.
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