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The mixture was thoroughly mixed by vortex mixing and incubated at 56 °C until the tissue was completely lysed.
The mixture was thoroughly mixed by vortex mixing and incubated at 50 °C inside a water bath until the tissue was completely lysed.
Five hundred microliters of 100-mM anhydrous disodium hydrogen phosphate was added to all samples and mixed by vortex.
Total lipid extract was dissolved in1.0 ml dry chloroform, and mixed by vortex.
Beads were resuspended in 250 μl 50 mM MES (pH 5.0) and mixed by vortex.
The mixture was mixed by vortex and dispensed (50 ul) in each tube.
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The mixture was mixed by vortex-shaking for 10 min and centrifuged at 13, 000 × g for 10 min. 10 μL of supernatant were applied to LC -MS/MS analysis.
The tube was thoroughly mixed by vortexing for 2 min.
After each incubation period, the samples were gently washed with sterile PBS, mixed by vortexing, and sonicated.
Five (5) g of REG-80, REG-GB, and REG-FFA were suspended in 5 mL water, mixed with 5 mL chloroform, and then mixed by vortexing.
Briefly, 25 μL of an aqueous solution of H2O210%% (v:v) was added to 250 μL of 1 M NaOH in a microcentrifuge tube and mixed by vortexing.
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