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BChE heterozygous mice [9] were maintained in a mixed background strain 129S1/SvImJ and B6D2.
Only male animals from the same mixed background strain generation were compared to each other.
Experiments using a mixed background strain may introduce noise into the data but the test for epistasis remains valid if the NntKO glucose intolerant phenotype is present.
Seventy-two female Tg2576 mice, over-expressing the human APP695 (Swedish) mutation, on a B6; SJL mixed background (strain #1349) (Hsiao et al., 1996) aged 10 12 weeks were purchased from Taconic and acclimatized until 26 weeks of age.
Similar(56)
Therefore, expression of the phenotype was seen in multiple generations on the mixed background strains used to create these mice.
Only animals from the same generation of the mixed-background strain were compared.
In addition to the mixed-background strain, Col1a1 r/r mice on a C57BL/6J background and 129P1/ReJ background were generated by backcrossing for at least 9 generations.
Additionally, we chose to work with a mixed background, to ensure no strain-specific effects.
Strains used were: a) FVB/N, and b) BALB/c Foxn1 nu / Foxn1 nu nude mice, c) MMTV-PyMT transgenic on FVB/N background, and d) Pten-deficient mice were in a mixed background of C57BL/6 and 129SvEvTac strain, in these mice inactivation of Pten has been achieved in several organs including breast using the Cre- loxP system (Pten loxP / loxP ; Mmtv-cre mice) [ 21, 22].
Myog-deleted mice were from a mixed background of C57/BL/6 and 129 strains.
All animals have a mixed background of 129SvJ and C57-Bl6/N streceivedeceived food and water ad libitum and lived in a light-dark cycle of 12 h.
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