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Thereafter, the standard frequency difference of the orbiter and the lander can be obtained from the mixed and transferred signals.
An equal amount of ZnO and graphite powders were mixed and transferred to an alumina boat inside the processing tube.
The two resulting solutions were thoroughly mixed and transferred into an evaporating dish and dried at 55 °C for 10 h.
The measured fluid velocity showed a complementary two-layer structure with the upper layer being dominated by a pair of counter-rotating vortices that kept this portion of the liquid well mixed and transferred heat from the hot pool wall to the pool center, while the flow in the lower layer was uniformly low in value and vertical.
The tube was vigorously mixed and transferred to a water bath at 95°C for 5 min for extraction of metabolites and inactivation of enzymes.
These clusters derived from some embryos were mixed and transferred onto a new feeder layer for propagation, because the cells needed to be high density to establish rES cell lines.
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Each sample mixture was vortex-mixed and transferred to a Centri-Free centrifugal filter unit (30,000 MWCO) and centrifuged at 1000 g for 30 minutes.
For each qRT-PCR reaction, 5 μl of EXPRESS SYBR GreenER qPCR SuperMix Universal (Invitrogen Life Technologies), 0.4 μl of 10 μM each of forward and reverse primers, 2.6 μl of nuclease-free water (Ambion) and 2 μl of diluted cDNA were pre-mixed and transferred into a LightCycler 480 Multiwell Plate 384 (Roche Diagnostics).
These are mixed vigorously and transferred to a glass insert inside a Kimble vial and 10 μL was injected onto the HPLC system.
Equimolar solutions of hexamethylenetetramine (99.9%, Sigma-Aldrich, St . Louis MO, USA) and zinc nitrate (Zn (NO3 2 · 6H2O) (99.9%, Sigma-Aldrich, St . Louis MO, USA) were mixed thoroughly and transferred to polymer autoclaves to serve as the precursors.
Alternatively 1 ml of TRI-reagent™ (Sigma) was added to each aRNA sample, mixed well and transferred to a PLG tube.
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