Sentence examples for mixed and subjected from inspiring English sources

PCR master mix (40  μl) was added to each well, mixed and subjected to thermal cycling with primer pair specific parameters.

Total RNA were mixed and subjected to reverse transcription with or without reverse transcriptase, followed by PCR amplification.

An appropriate amount of urea, glucose, and pretreated CNTs was mixed and subjected to hydrothermal treatment at 180 °C for 6 h.

25 µg of each individual labeled samples (control, UVB-treated and pooled standard samples from triplicate experiments) were mixed and subjected to isoelectric focusing on a IPGphor isoelectric focusing unit (GE Healthcare, Uppsala, Sweden) along a continuous 4 7 pH gradient using IPG strips (18 cm, linear 4 7 pH, GE Healthcare, Uppsala, Sweden) with details in supplementary materials.

Purified in vitro components were mixed and subjected to the virion assembly assay, as previously described.

Equal molar ratios of purified SOD2 proteins and internal control peptides were labelled by iTRAQ labelling reagents (ABI), mixed and subjected to LTQ-OrbiTrap analysis under PQD mode.

Pterygia and conjunctiva tissues containing 100 μg of protein were mixed with an equal volume of buffer and then boiled for 5 min. Samples from human serum were dissolved in 15 μl of sample preparation buffer, mixed well, and subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis according to Laemmli protocol [ 17].

This was repeated in two sub-samples excluding either patients with mixed pain only or patients with mixed and subjects with central NP (just to test the instrument in patients with peripheral NP only).

The samples were mixed and then subjected to intact protein fractionation by ion exchange followed by reverse phase chromatography.

The DP were 1) MET: digesta was incubated at 38°C for 15 min with saline solution (0.9% NaCl) containing 0.1% methylcellulose under continuous shaking; 2) STO: digesta was mixed with cold saline solution and homogenized with a stomacher for 5 min at 230 rpm; 3) FRE: digesta was immediately frozen at −20°C for 72 h, thawed at 4°C, mixed with saline solution and subjected to STO procedure.

The fbsA-flanking PCR products were mixed in equal amounts and subjected to a crossover PCR with the primers fbsA _del1 and fbsA _del4, resulting in one PCR product that carried the two fbsA-flanking regions.