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They were mixed and spun down for 5 minutes at 2,000 rpm.
After 20E was added, the media were well mixed and spun down a day before use.
An equal volume of isopropanol was added, mixed, and spun for 20 min at 12,000 × g and 4°C.
Subsequently 200 μl of a saturated carbonate solution were added, mixed and spun down for 10 min at 3000 rpm.
After fragmentation, 57 μl of 2XGEx hybridisation buffer (Agilent) was added to each sample which was then briefly mixed and spun down before being stored on ice in preparation for loading 103 μl onto each microarray.
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The fibres are mixed with polyester and spun into yarn.
At the conference, Dr. Philippe Poulin, a scientist at the National Center for Scientific Research in France, described how nanotubes could be dispersed into a liquid, mixed with polymers and spun into a fiber thinner than a human hair.
The lysates were vigorously mixed with chloroform and spun down at 12000×g for 20 minutes at 4°C.
In Pr0.55(Ca1 − ySr y )0.45MnO3, Mn4+ d3, S = 3/2) and Mn3+ d4, S = 2) configurations are mixed, and spin-charge-orbital orderings are expected.
Then, 7.0 wt.% PMMA and 2.8 wt.% FR solutions were mixed and spin-coated onto freshly cleaned silicon wafers (crystallographic orientation (100), resistance 0.002 Ω cm, refractive index n = 3.50) or glass substrates (supplied by Glassbel Ltd., Prague, Czech Republic).
The reactions were gently mixed and briefly spun down and run in a PTC-100™ MJ PTC-100™ MJ Research Incitions at 16°C for 30 min, 42°C for 30 min, and 85°C for 5 min. For the real-time PCR, the TaqMan® 2× Universal PCR Master Mix, No AmpErase® UNG withused (Applied Biosystems, PN 4324020).
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